Question first qs are q4 that mentioned in second qs please solve them with explanations The following in vitro experiment was performed with isolated mitochondria in an aerobic buffer. The mitochondrial suspension was supplied with B-hydroxybutyrate (BHB), ADP and Pi. As the experiment progressed, oxygen was consumed and ATP was synthesized. Mitochondria have an enzyme inside the matrix that catalyzes the oxidation of BHB in the following manner: CH Enzyme ? NADH+H H-C-OH CH, NAD B-hydroxybutyrate (BHB) (a) How many electrons and protons are transferred to NAD from BHB? (b) Predict the structure of the product of the above enzymatic reaction. (c) BHB is routinely used in experiments to determine the P:O ratio for NADH oxidation. The product of the above reaction is a terminal product (meaning, nothing further happens to the product). Suggest reasons why working with BHB might be more useful than adding NADH directly to solutions of isolated mitochondria to determine P:O ratio for NADH oxidation? (d) How many moles of ATP are expected to be synthesized per mole of BHB oxidized? Predict the result of the following experiments by completing the accompanying graphs. (a) NADH is added (as the only donor) to a suspension of intact purified mitochondria in an aerobic buffer (pH 7.0) containing ADP and Pi and supplemented Cyto C NADH added Po] Absorbance at 340nm NADH added Time Time (b) NADH is added (as the only donor) to a suspension of inverted vesicles in an aerobic buffer (pH7.0) containing ADP & Pi and supplemented Cyto C NADH added Fo] Time (c) with B-hydroxybutyrate (B-HB), introduced in Q4, is added to suspension of intact purified mitochondria in an aerobic buffer (pH 7.0) containing ADP and Pi and supplemented Cyto C(use all the information from question 4 for this) B-HB added Absorbance by NADH at 340nm Pol 11 B-HB added Time Time

UGIGLE The Asker · Biology

first qs are q4 that mentioned in second qs
please solve them with explanations

Transcribed Image Text: The following in vitro experiment was performed with isolated mitochondria in an aerobic buffer. The mitochondrial suspension was supplied with B-hydroxybutyrate (BHB), ADP and Pi. As the experiment progressed, oxygen was consumed and ATP was synthesized. Mitochondria have an enzyme inside the matrix that catalyzes the oxidation of BHB in the following manner: CH Enzyme ? NADH+H H-C-OH CH, NAD B-hydroxybutyrate (BHB) (a) How many electrons and protons are transferred to NAD from BHB? (b) Predict the structure of the product of the above enzymatic reaction. (c) BHB is routinely used in experiments to determine the P:O ratio for NADH oxidation. The product of the above reaction is a terminal product (meaning, nothing further happens to the product). Suggest reasons why working with BHB might be more useful than adding NADH directly to solutions of isolated mitochondria to determine P:O ratio for NADH oxidation? (d) How many moles of ATP are expected to be synthesized per mole of BHB oxidized? Predict the result of the following experiments by completing the accompanying graphs. (a) NADH is added (as the only donor) to a suspension of intact purified mitochondria in an aerobic buffer (pH 7.0) containing ADP and Pi and supplemented Cyto C NADH added Po] Absorbance at 340nm NADH added Time Time (b) NADH is added (as the only donor) to a suspension of inverted vesicles in an aerobic buffer (pH7.0) containing ADP & Pi and supplemented Cyto C NADH added Fo] Time (c) with B-hydroxybutyrate (B-HB), introduced in Q4, is added to suspension of intact purified mitochondria in an aerobic buffer (pH 7.0) containing ADP and Pi and supplemented Cyto C(use all the information from question 4 for this) B-HB added Absorbance by NADH at 340nm Pol 11 B-HB added Time Time
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Transcribed Image Text: The following in vitro experiment was performed with isolated mitochondria in an aerobic buffer. The mitochondrial suspension was supplied with B-hydroxybutyrate (BHB), ADP and Pi. As the experiment progressed, oxygen was consumed and ATP was synthesized. Mitochondria have an enzyme inside the matrix that catalyzes the oxidation of BHB in the following manner: CH Enzyme ? NADH+H H-C-OH CH, NAD B-hydroxybutyrate (BHB) (a) How many electrons and protons are transferred to NAD from BHB? (b) Predict the structure of the product of the above enzymatic reaction. (c) BHB is routinely used in experiments to determine the P:O ratio for NADH oxidation. The product of the above reaction is a terminal product (meaning, nothing further happens to the product). Suggest reasons why working with BHB might be more useful than adding NADH directly to solutions of isolated mitochondria to determine P:O ratio for NADH oxidation? (d) How many moles of ATP are expected to be synthesized per mole of BHB oxidized? Predict the result of the following experiments by completing the accompanying graphs. (a) NADH is added (as the only donor) to a suspension of intact purified mitochondria in an aerobic buffer (pH 7.0) containing ADP and Pi and supplemented Cyto C NADH added Po] Absorbance at 340nm NADH added Time Time (b) NADH is added (as the only donor) to a suspension of inverted vesicles in an aerobic buffer (pH7.0) containing ADP & Pi and supplemented Cyto C NADH added Fo] Time (c) with B-hydroxybutyrate (B-HB), introduced in Q4, is added to suspension of intact purified mitochondria in an aerobic buffer (pH 7.0) containing ADP and Pi and supplemented Cyto C(use all the information from question 4 for this) B-HB added Absorbance by NADH at 340nm Pol 11 B-HB added Time Time
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Answer - Oxidation of beta hydroxybutarate gives -Acetoacetate(ketone) and then the final product is ACETONECS Scanned with CarmScannerAnswer 1(a) - in oxidation of beta hydroxybutarate one electronis transferred to NAD hence act as an electron donor.Answer1(b) same as in picture attached i.e. Acetoacetate , alsofinal product of this reaction is ACETONEAnswer 1( ... See the full answer