Question You measure distances from the center of a diffraction pattern (y) to a series of dark fringes on a screen that is 0.3000 ± 0.0005 m away from the 0.04-mm wide slit you are using to create the pattern. You create a plot of y (in m) vs m and get a slope for the best-fit line of 0.005245 ± 8.575 10-6. What is the wavelength of the laser you used to collect the data? Please include the answer in regular notation. Thank you

A charge of 6 x 10^-7 C is transferred from infinity to a point B in an electric field. If the work done by the electric force to do this is 1.2 x 10^-5 J, what is the potential at point B? (Also, draw the appropriate vector diagram with labels)

A) Which protein bands in the autoradiograph ( Figure B ) Correspond to cadherin and which correspond to a protein that binds to cadherin? Explain your answer ( 2 marks ) B) Which segment of cadherin is responsible for binding to other proteins? ( 2 marks ) C) with what is known about cadherin complexes, speculate on the possible identity of the immunoprecipitated proteins ( 1 mark) Question 2 Not yet answered Marked out of 5.00 A researcher is interested in the proteins that E-cadherin binds to. She expresses full-length E-cadherin as well some truncated constructs of the protein in a mouse cell line that does not express any other cadherins. The scheme of constructs is shown in figure A. (E represents the extracellular domain, TM the transmembrane domain and C the cytoplasmic domain). She labels the cells with 355 methionine for 16 hours, homogenizes them in detergent, and an antibody raised against the extracellular domain of the cadherin E-cacherin to pull-down (co- immunoprecipitate) E-cadherin and any associated proteins. She then analyses the immune precipitates by electrophoresis by SDS-PAGE, followed by autoradiography to make the labelled proteins visible. In lane 1 of figure B, the antibody precipitates three major proteins in cells transfected with a plasmid expressing the full-length intact cadherin. These proteins are not precipitated from untreated control cells. E p Flag question H2N A TM C COOH 553aa +24 aa 151 a. Full-length 37 aa E-Cad A37 TTTT 18 E-Cad 48 31 aa Tii E-Cad A31 70 a.a E-Cad A70 105 aa E-Cad A105 B kd 200 Full-length E-Cad 137 E-Cad 18 E-Cad A31 E-Cad 170 E-Cad 105 control 97 - 67 - 45 - 1 2 3 4 5 6 7

A researcher is interested in the proteins that E-cadrerin binds to. She expresses full-length E-cadherir as well some truncated constructs of the protein in a mouse cell line that does not express any other cadherins. The scheme of constructs is shown in figure A (E represents the extracelular comair, TM the transmembrane domain and C the cytoplasmic A researcher is interested in the proteins that E-cadrerin binds to. She expresses full-length E-cadherir as well some truncated constructs of the protein in a mouse cell line that does not express any other cadherins. The scheme of constructs is shown in figure A (E represents the extracelular comair, TM the transmembrane domain and C the cytoplasmic domain). She labels the cells with 35 methionine for 16 hours, homogenizes them in detergent, and an antibody raised against the extracellu ar domain of the cacherin C-cacherin to pull-down (co- immunoprecipitate) C-cadherin and any associated proteins. She then analyses the immune precipitates by electrophoresis by SDS-PAGC, followed by autoradiography to make the labelled proteins visible. In lane 1 of figure B, the antibody precipitates three major proteins in cells transfected with a pasmid expressing the full-length intact cadherin. These proteins are not precipitated from untreated control cells. H₂N E TM C A COOH 553aa 24 a aa m B kd 200 - 97 - 67- 45- www. m Full-length 31 aa 70 aa 105 aa 151 aa 37aa E-Cad 437 E-Cad 48 E-Cad 431 E-Cad 470 E-Cad 105 control 1 2 3 4 5 6 7 Full-length E-Cad 437 E-Cad 48 E-Cad 431 E-Cad 470 E-Cad A105 a. Which protein bands in the autoradiograph (Figure B) correspond to cacherir and which correspond to proteins that bind to cadherin? Explain your answer. (2 marks) b. Which segment of cadherin is responsible for binding to other proteins? (2 marks) c. With what is known about cadherin complexes, speculate on the possible identity of the immunoprecipitated proteins. (1 mark)

Pelcher Company maintains a $425 petty cash fund. On January 31, the fund is replenished. The accumulated receipts on that date represent $115 for office supplies, $150 for merchandise inventory, and $75 for miscellaneous expenses. There is a cash coverage of $9. Based on the information, the amount of cash in the fund replenishment

Exercise 5.13: Consider the relation CLASS(Course#, Univ_Section#, Instructor_name, Semester, Building_code, Room#, Time_period, Weekdays, Credit_hours). This represents classes taught in a university, with unique Univ_section#s. Identify what you think should be various candidate keys, and write in your own words the conditions or assumptions under which each candidate key would be valid. Exercise 5.14: Consider the following six relations for an order-processing database application in a company: CUSTOMER(Cust#, Cname, City) ORDER(Order#, Odate, Cust#, Ord_amt) ORDER_ITEM(Order#, Item#, Qty) ITEM(Item#, Unit_price) SHIPMENT(Order#, Warehouse#, Ship_date) WAREHOUSE(Warehouse#, City) Here, Ord_amt refers to total dollar amount of an order; Odate is the date the order was placed; and Ship_date is the date an order (or part of an order) is shipped from the warehouse. Assume that an order can be shipped from several warehouses. Specify the foreign keys for this schema, stating any assumptions you make. What other constraints can you think of for this database? Perform exercises using a word document with diagrams or other items pasted in if necessary. Be sure to paraphrase and included any sources used in finding solutions. Submit to dropbox.

Calculate the linear combination. 9(i + 2)) - 3(2j+ k) + 7(26 - 1) = (x, y, z) (Give your answers as a whole or exact number.) y =