**QUESTION**

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compound extracted from the cottee, you have discovered that it is an inhibitor of $\mathrm{X} Y$ /ase. Given this revelation, it seems clear that this compound is the most likely murder weapon. The data you have gathered is shown in Table 1. Table 1 \begin{tabular}{|c|c|c|} \hline [S] (nM) & $\mathbf{V}_{\mathbf{0}}$ (no inhibitor) & $\mathbf{V}_{\mathbf{0}}$ (Compound $\left.\mathbf{X}\right)$ \\ \hline 50 & 0.14 & 0.040 \\ \hline 75 & 0.19 & 0.050 \\ \hline 150 & 0.32 & 0.080 \\ \hline 400 & 0.47 & 0.14 \\ \hline \end{tabular} Note: [S] is given in $\mathrm{nM}$; other values represent observed $\mathrm{V}_{0}$ values given in $\mu \mathrm{mol} / \mathrm{min}$ of product formed. Use the data collected to create Michaelis-Menten and Lineweaver-Burk plots with and without Compound $\mathrm{X}$ present. From the limited data you have gathered, does it appear that XYZase follows Michaelis-Menten kinetics? yes no Now calculate the Michaelis constant, $K_{\mathrm{m}}$, of $\mathrm{XYZ}$ ase within two significant figures. \[ K_{\mathrm{m}}= \]